Detection of bluetongue virus and African horsesickness virus in co-infected cell cultures with NS1 gene probes.

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

Characterization of tubular structures composed of nonstructural protein NS1 of African horsesickness virus expressed in insect cells.

The characteristic tubules that are produced during the orbivirus infection cycle are composed of a major viral nonstructural protein, NS1. To characterize the NS1 gene and gene product of African horsesickness virus (AHSV), a full-length cDNA copy of the NS1 gene of AHSV-6 was cloned and the nucleotide sequence determined. NS1 was highly conserved within the AHSV serogroup with between 95-98% ...

On the relationship between bluetongue, African horsesickness and reoviruses: hybridization studies.

VERWOERD, D. W. & HUISMANS, H . On the relationship between bluetongue, Afr ican horsesickness and reo viruses : hybridization studies. Onderstepoort J. vet. Res., 36 (2) , 175-180, 1969. The double-stranded ribonucleic acid from bluetongue virus (BTV) , African horsesickness virus (AHSV) and reovirus has been tested for hybridization with messe~ger RNA derived from BTV and reovirus-infected ce...

The complete sequence of African horsesickness virus serotype 4 (vaccine strain) RNA segment 5 and its predicted polypeptide compared with NS1 of bluetongue virus.

The complete sequence of RNA segment 5 of the African horsesickness virus serotype 4 (AHSV-4) vaccine strain was determined from cDNA clones inserted into pBR322. The RNA is 1751 bp long (M(r) 1.12 x 10(6)) and contains an open reading frame encoding a protein of 548 amino acids (M(r) 63,122) with a net charge of +0.5 at neutral pH. A comparison of the sequence of AHSV-4 segment 5 with that of ...

Applying conserved peptides of NS1 Protein of avian influenza virus to differentiate infected from vaccinated chickens

Avian influenza (AI) is a highly contagious disease in poultry and outbreaks can have dramatic economic and health implications. For effective disease surveillance, rapid and sensitive assays are needed to detect antibodies against AI virus (AIV) proteins. In order to support eradication efforts of avian influenza (AI) infections in poultry, the implementation of “DIVA” vaccination strategies, ...